The isolation and regeneration of potato (Solanum tuberosum L) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid . The Isolation of regeneratiom of potato (Solanum tuberosum L.) protoplasts have been carried out. Mesophyl cell protoplast were isolated from two dihaploid. One of new technologies in order to create hybrid plant from different species is protoplast fusion. This technique involves protoplast isolation, purification.

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Callus wich has been two times resubcultured was suspended in the digestive medium without driselase, CaCl2, and MES and incubated in lowlight isolask by constant shaking at rpm in reciprocal water shaker at 30 celcius centigrade.

Please provide a name for this query: Get permanent URL for this record. They were incibated in full darkness at 27 Celcius centigrade by constant shaking at 50 rpm orbitor shaker. Our members Our publisher members Our sponsors Our volunteers.


Regeneration of cell wall observed progessively in mother protoplast from tobacco, cowpea A27, E1, and H6 and fused protoplast of soybean with tobacco, tobacco with cowpea C4, E1, and H6soybean with cowpea C4 and between cowpea C4 and cowpea E1.


Indonesian, English Full-text formats available: Indonesia Language of fulltext: The results showed protkplas there was effect of treatment for all variables observed. Close Go to Workspace. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0. Publication Year Publication Year. Results 1 – 1 of 1.

Isolasi Protoplas secara Enzimatis pada Tanaman Kecipir | Riyadi | Buletin Plasma Nutfah

Protoplast were collected by centrifugation of g and a thin layer of the suspension was irradiated with ultraviolet light. The first factor was concentrations of sucrose which consists of three levels namely 0. The obyectives of the research was to investigate the effect of combination treatment of isolaai concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii. Isolation of protoplast from soybean, cowpea, and tobacco and their fusion.

Agriculture General Country of publisher: Protoplast were cultured on the medium of Ikeda and Uchimia.

Javascript must be enabled for narrowing. Standard Search Advanced Search. Cell wall generation was observed.

Looks like Javascript is disabled on your browser. Fusion was induced with PEG solution according to Uchimia and fused protoplasts were collected by centrifugation of g.


Journal on Agriculture Science. Funding for sustainable Open Access incl. Reference Number Reference Number.

Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. For soybean leaf, the medium was enriched with driselase and pectolyase Y Journal Homepage Journal Title: Leaf protoplast were releasaed in h, soybean and tobacco callus protoplast in h, and cowpeas callus protoplast in h of incubation.

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Isolasi dan fusi protoplas dari kedelai, buncis, dan tembakau. On both medium leaf protoplast, irradiated protoplasts and their fused do not regenerate cell wall and all cultured died out within four weeks incubation.

The factorial experiment consisted of two factors that was arranged by completely randomized design CRD with ten replications.